Journal: Clinical and Vaccine Immunology : CVI

Article Title: Salmonella enterica Serovar Enteritidis Ghosts Carrying the Escherichia coli Heat-Labile Enterotoxin B Subunit Are Capable of Inducing Enhanced Protective Immune Responses

doi: 10.1128/CVI.00016-14

Figure Lengend Snippet: Bacterial strains, plasmids, and primers utilized in this study

Article Snippet: TABLE 1 Strain, plasmid, or primer Description Reference or source Bacterial strains E. coli F − ompT hsdS B (rB − mB − ) dcm gal λ(DE3) pLysS Cmr Promega BL21(DE3)pLysS JOL500 Wild-type F18 + , LT + , STa + , STb + , stx 2 + , stx 2e + ETEC isolate from pig Lab stock S . Enteritidis JOL1254 asd gene knockout strain Lab stock JOL1182 Wild type Lab stock JOL1373 JOL1254 containing pMMP172 Lab stock JOL1358 JOL1254 containing pJHL187-LTB This study JOL860 Wild-type isolate from chickens Lab stock Plasmids pMMP172 asd + pBR ori plasmid carrying ghost cassette 19 pET28a IPTG-inducible expression vector; Km r Novagen pET28a-LTB pET28a derivative containing eltB Present study pYA3342 asd + vector, pBR ori 44 pYA3332 asd + vector; p15A ori 44 pJHL187 asd + p15A ori plasmid carrying ghost cassette This study pJHL187-LTB pJHL187 containing eltB gene This study Primers eltB-F 5′-CCGCGAATTCGCTCCCCAGTCTATTACAG-3′ 39 eltB-R 5′-CCGCAAGCTTCTAGTTTTCCATACTGATTG-3′ ompA-F-NcoI 5′-CCATGGATGAAAAAGACAGCTATCGC-3′ This study OmpA-E/K/H-H6-Sal-R 5′-TAAGTCGACATGATGATGATGATGATGAAGCTTGGTACCGAATTCCAGACGGGTAGCGAT-3′ Open in a separate window Bacterial strains, plasmids, and primers utilized in this study.

Techniques: Plasmid Preparation, Gene Knockout, Expressing

Journal:

Article Title: Methionine Sulfoxide Reduction and Assimilation in Escherichia coli : New Role for the Biotin Sulfoxide Reductase BisC

doi: 10.1128/JB.187.1.231-237.2005

Figure Lengend Snippet: Strains and plasmids

Article Snippet: Mutations were transferred between different strains by transduction with phage P1 (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant characteristics Source or reference Strains DH5α φ80d lacZ Δ M15 recA1 endA1 gyr A96 thi-1 hsdR17 supE44 relA1 deoR Δ( lacZYA-argF ) U169 Promega BL21 (DE3) F − ompT hsdS (r B − m B − ) gal dcm (DE3) Novagen BW25113 lacI q rrnB T14 Δ lacZ WJ16 hsdR514 Δ araBAD AH33 Δ rhaBAD LD78 2 MC4100 araD139 Δ ( argF-lac ) U169 rpsL150 relA1 thi fibB5301 deoC1 ptsF25 rbsR Promega HMS174 (DE3) F − recA hsdR (r K 12 − m K 12 + ) Rif r (DE3) Novagen LCB303 MC4100 metB 1 Lab collection BE54 MC4100 metB 1 msrA ::Spc r msrB ::Cm r 7 Tp1000 MC4100 Δ mobAB Kan r 18 BE72 MC4100 metB 1 msrA ::Spc r msrB ::Cm r Δ mobAB ::Kan r This study JB02 MC4100 metB 1 msrA ::Spc r This study JB07 BW25113 bisC ::Kan r This study JB08 MC4100 metB 1 msrA ::Spc r msrB ::Cm r bisC ::Kan r This study JB09 MC4100 metB 1 bisC ::Kan r This study JB11 MC4100 metB 1 trxA ::Kan r This study JB308 MC4100 metB 1 msrA ::Spc r msrB ::Cm r bisC ::Kan r / pBis2 This study JB309 MC4100 metB 1 msrA ::Spc r msrB ::Cm r bisC ::Kan r / pBis1 This study Plasmids pJBis pET21a+, BisCorf2 (His 6 tag), Amp r This study pBis1 pBad24, BisC(orf1), Amp r This study pBis2 pBad24, BisC(orf2), Amp r This study pMsrA pUC18, MsrA Amp r 7 pKD46 Amp r 2 pKD4 Kan r 2 Open in a separate window Strains and plasmids Plasmid construction.

Techniques: Plasmid Preparation

Journal: Metabolic engineering

Article Title: De novo production of benzyl glucosinolate in Escherichia coli.

doi: 10.1016/j.ymben.2019.02.004

Figure Lengend Snippet: Figure 4. Medium levels of dsBGLS and BGLS produced by pRI952 E. coli strain grown in

Article Snippet: Strain Name used Characteristics Reference BL21 (DE3) BL21 (DE3) pLysS RosettaTM (DE3)pLysS BL21 (DE3) with pRI952 BL21 (DE3) pLysS Rosetta pRI952 Genome integrated placUV5::T7 RNA polymerase BL21 (DE3) containing plasmidexpressed T7 lysozyme pLysS including rare tRNAs on plasmid BL21 (DE3) with rare tRNAs on NEB® C2527I Promega L1191 Novagen®, Merck 70956 Del Tito et al. 1995 M AN US CR IP T AC CE PT ED One Shot® BL21-AITM NEB® 5-alpha with pTARA1 NEB® 10-beta with pTARA1 BL21-AI DH5a DH10B plasmid Genome integrated paraBAD ::T7 RNA polymerase Cloning strain with plasmidexpressed paraBAD::T7 RNA polymerase Cloning strain with plasmidexpressed paraBAD::T7 RNA polymerase ThermoFisher C6070-03 NEB® C2987H NEB® C3019H 1pTARA plasmid constructed in (Wycuff & Matthews, 2000).

Techniques: Produced

Journal: Metabolic engineering

Article Title: De novo production of benzyl glucosinolate in Escherichia coli.

doi: 10.1016/j.ymben.2019.02.004

Figure Lengend Snippet: Figure 5. Production of dsBGLS and BGLS in medium from pRI952 E. coli strain upon 453

Article Snippet: Strain Name used Characteristics Reference BL21 (DE3) BL21 (DE3) pLysS RosettaTM (DE3)pLysS BL21 (DE3) with pRI952 BL21 (DE3) pLysS Rosetta pRI952 Genome integrated placUV5::T7 RNA polymerase BL21 (DE3) containing plasmidexpressed T7 lysozyme pLysS including rare tRNAs on plasmid BL21 (DE3) with rare tRNAs on NEB® C2527I Promega L1191 Novagen®, Merck 70956 Del Tito et al. 1995 M AN US CR IP T AC CE PT ED One Shot® BL21-AITM NEB® 5-alpha with pTARA1 NEB® 10-beta with pTARA1 BL21-AI DH5a DH10B plasmid Genome integrated paraBAD ::T7 RNA polymerase Cloning strain with plasmidexpressed paraBAD::T7 RNA polymerase Cloning strain with plasmidexpressed paraBAD::T7 RNA polymerase ThermoFisher C6070-03 NEB® C2987H NEB® C3019H 1pTARA plasmid constructed in (Wycuff & Matthews, 2000).

Techniques:

Journal: Metabolic engineering

Article Title: De novo production of benzyl glucosinolate in Escherichia coli.

doi: 10.1016/j.ymben.2019.02.004

Figure Lengend Snippet: Figure 6. Protein levels in pRI952 E. coli strain upon optimisation of CYP and SOT activity. 476

Article Snippet: Strain Name used Characteristics Reference BL21 (DE3) BL21 (DE3) pLysS RosettaTM (DE3)pLysS BL21 (DE3) with pRI952 BL21 (DE3) pLysS Rosetta pRI952 Genome integrated placUV5::T7 RNA polymerase BL21 (DE3) containing plasmidexpressed T7 lysozyme pLysS including rare tRNAs on plasmid BL21 (DE3) with rare tRNAs on NEB® C2527I Promega L1191 Novagen®, Merck 70956 Del Tito et al. 1995 M AN US CR IP T AC CE PT ED One Shot® BL21-AITM NEB® 5-alpha with pTARA1 NEB® 10-beta with pTARA1 BL21-AI DH5a DH10B plasmid Genome integrated paraBAD ::T7 RNA polymerase Cloning strain with plasmidexpressed paraBAD::T7 RNA polymerase Cloning strain with plasmidexpressed paraBAD::T7 RNA polymerase ThermoFisher C6070-03 NEB® C2987H NEB® C3019H 1pTARA plasmid constructed in (Wycuff & Matthews, 2000).

Techniques: Activity Assay

Journal: Journal of Bacteriology

Article Title: Distinct Regulatory Role of Carbon Catabolite Protein A (CcpA) in Oral Streptococcal spxB Expression

doi: 10.1128/JB.00619-17

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: The following antibiotics were supplemented for selection: kanamycin (100 μg ml −1 ), erythromycin (10 μg ml −1 ), and spectinomycin (200 μg ml −1 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant characteristic(s) Reference or source S. gordonii DL1 Wild type 64 S. sanguinis SK36 Wild type ATCC BAA-1455 S. mitis 12261 Wild type 65 S. oralis J22 Wild type 66 S. parasanguinis Wild type, clinical isolate 25 S. infantis Wild type, clinical isolate 25 S. gordonii DL1 Δ ccpA ccpA deletion mutant ( ccpA :: ermAM ) 31 S. sanguinis SK36 Δ ccpA ccpA deletion mutant ( ccpA :: ermAM ) 35 S. gordonii DL1 Δ spxB spxB deletion mutant ( spxB :: ermAM ) 67 S. sanguinis SK36 Δ spxB spxB deletion mutant ( spxB :: ermAM ) 67 S. gordonii DL1 ccpA :: ccpA (SK36) Expression of heterologous CcpA from SK36, Spc r This study S. gordonii DL1 spxB p(SK36) - spxB spxB expression under the control of hererologous spxB promoter of SK36, Spc r This study S. gordonii DL1 spxB p(DL1) - luc spxB luciferase reporter (natural spxB promoter), Spc r This study S. gordonii DL1 spxB p(SK36) - luc spxB luciferase reporter (heterologous spxB -promoter of SK36), Spc r This study S. sanguinis SK36 ccpA :: ccpA (DL1) Expression of heterologous CcpA from DL1, Spc r This study S. sanguinis SK36 spxB p(DL1) - spxB spxB expression under the control of heterologous spxB -promoter of DL1, Spc r This study S. sanguinis SK36 spxB p(SK36) - luc spxB luciferase reporter (natural spxB -promoter), Spc r This study S. sanguinis SK36 spxB p(DL1) - luc spxB luciferase reporter (heterologous spxB -promoter of DL1), Spc r This study E. cloni 10G E. coli cloning strain, Amp r Lucigen, WI E. coli BL21(DE3) pLysS Protein expression strain, Cpm r Promega, WI BL21(DE3) pLysS(pET29b(+) + Sg CcpA ) Expression of CcpA of DL1, Kan r Cpm r This study BL21(DE3) pLysS(pET29b(+) + Ss CcpA ) Expression of CcpA of SK36, Kan r Cpm r This study pDL 278 E. coli - Streptococcus shuttle vector, Spc r 68 pET 29b(+) N-terminal 6× His fusion protein expression vector; Kan r Novagen/Milliore Sigma, MA pFW5-luc Suicide vector; Spc r 69 Open in a separate window Strains and plasmids used in this study DNA manipulations.

Techniques: Plasmid Preparation, Mutagenesis, Expressing, Control, Luciferase, Cloning